|
KMID : 1145120190170030287
|
|
Asian Journal of Beauty and Cosmetology
2019 Volume.17 No. 3 p.287 ~ p.294
|
|
|
Whitening effect of Brazilin in B16F10 cells
|
|
Shim Joong-Hyun
|
|
|
Abstract
|
|
|
Purpose: This study aimed to identify the whitening effect of brazilin on the B16F10 cell line.
Method: I measured the cell viability, mRNA expression, tyrosinase activity inhibition assay, and melanin contents assay from B16F10 cells. Also, I elucidated the effects of brazilin on tyrosinase-related protein 1 (TYRP1)/TYRP2/ tyrosinase (TYR)/microphthalmia-associated transcription factor (MITF) mRNA expression, and melanin production.
Results: Quantitative real-time RT-PCR showed that brazilin decreased the mRNA level of TYRP1/TYRP2/TYR/MITF and melanin production compared with that of the MSH-treated B16F10 cells. The tyrosinase activity inhibition assay revealed that brazilin decreased melanin production in B16F10 cells.
Conclusion: These data show that brazilin increases whitening effects in B16F10 cells and thus brazilin is a potent ingredient for skin whitening. Future studies based on these results can determine the potential uses of brazilin in cosmetics, health care, food, or medicine.
|
|
|
KEYWORD
|
|
|
Brazilin, Whitening, Melanin, TYR, MITF
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|
|